Rapid and reliable cloning of PCR products.
نویسنده
چکیده
منابع مشابه
Enzyme-free cloning: a rapid method to clone PCR products independent of vector restriction enzyme sites.
We describe a simple method for the cloning of PCR products without the need for post-amplification enzymatic treatment. Tailed PCR primer sets are used to create complementary staggered overhangs on both insert and vector by a post-PCR denaturation-hybridisation reaction. The single-stranded overhangs are designed to allow directional cloning in a ligase-free manner. This 'enzyme-free cloning'...
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products. BioTechniques 12:28-30. 10.Scharf, S.J., G.T. Horn and H.A. Erlich. 1986. Direct cloning and sequence analysis of enzymatically amplified genomic sequences. Science 233:1076-1078. 11.Shuldiner, A.R., L.A. Scott and J. Roth. 1990. PCR-induced (ligase free) subcloning: a rapid reliable method to subclone polymerase chain reaction (PCR) product. Nucleic Acids Res. 18:1920. 12.Starr, L. a...
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A rapid DNA cloning system is a research interest of many scientists. TA cloning is one of the methods used for the cloning of PCR-amplified DNA molecules. The TA cloning method is a convenient and labor-saving replacement to traditional, restriction enzyme-mediated cloning strategies. A T-vector called pBlueskript ΙΙ SK-1 with the lethal gene ccdB was designed to construct a positive selection...
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ورودعنوان ژورنال:
- PCR methods and applications
دوره 1 2 شماره
صفحات -
تاریخ انتشار 1991